TY - BOOK AU - Ninfa, Alexander J. AU - Ballou,David P. AU - Benore,Marilee AU - Krylova,L. TI - Fundamental laboratory approaches for biochemistry and biotechnology SN - 9780470087664 U1 - 660.6078 PY - 2010/// CY - Hoboken, N.J. PB - John Wiley & Sons, Inc. KW - Biochemical Phenomena KW - Biotechnology KW - Biochemistry N1 - 1. Getting started in scientific research. --2. Basic procedures in the biochemistry laboratory.-- 3. Spectroscopic methods. -- 4. Quantification of protein concentration. -- 5. Chromatography. -- 6. Gel Electrophoresis of protein. -- 7. Overview of protein purification.-- 8. Subcellular fractionation. -- 9. Isolation & characterization of an enzyne - alkaline phosphatase from escherichia coli. -- 10. Ensyme kinetics. -- 11. Ligand binding. -- 12. Enzymatic methods of analysis. -- 13.Recombinant DNA techniques.-- 14. Polymerase chain reaction (PCR) technology.-- 15. Using the computer & the internet for biochemical research & communication ER -