Fundamental laboratory approaches for biochemistry and biotechnology :
- 2nd ed
- Hoboken, N.J. : John Wiley & Sons, Inc. c2010.
- xix, 458 p. : ill. ;
1. Getting started in scientific research. --2. Basic procedures in the biochemistry laboratory.-- 3. Spectroscopic methods. -- 4. Quantification of protein concentration. -- 5. Chromatography. -- 6. Gel Electrophoresis of protein. -- 7. Overview of protein purification.-- 8. Subcellular fractionation. -- 9. Isolation & characterization of an enzyne - alkaline phosphatase from escherichia coli. -- 10. Ensyme kinetics. -- 11. Ligand binding. -- 12. Enzymatic methods of analysis. -- 13.Recombinant DNA techniques.-- 14. Polymerase chain reaction (PCR) technology.-- 15. Using the computer & the internet for biochemical research & communication.